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R&D Systems™ ProDots Human BMP-4 Recombinant Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.
Supplier: R&D Systems™ PRD31401
This item is not returnable.
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Description
The ProDots Recombinant Human BMP-4 Protein is derived from NS0. The ProDots Recombinant Human BMP-4 Protein has been validated for the following applications: Bioactivity.Specifications
Q53XC5 | |
652 | |
5μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution. | |
BMP2B, BMP-2B, BMP2B1, BMP2BMCOPS6, BMP4, BMP-4, Bone morphogenetic protein 2B, bone morphogenetic protein 4, DVR4, MCOPS6, OFC11, ZYME | |
BMP4 | |
Unconjugated | |
Recombinant | |
Mouse myeloma cell line, NS0-derived Ser293 - Arg408 |
Bioactivity | |
Observed M.W.: 22-25kDa, reducing conditions 37-41, non-reducing conditions, Theoretical M.W.: 13kDa (monomer) | |
Mouse myeloma cell line,NS0-derived human BMP-4 protein Ser293-Arg408 | |
<0.01 EU per 1μg of the protein by the LAL method. | |
BMP-4 | |
Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. Binnerts, M.E. et al. (2004) Biochem. Biophys. Res. Commun. 315(2):272. The ED50 for this effect is typically 2.5-15.0ng/mL. | |
Human | |
For a stock solution, reconstitute at 50-200 μg/mL in sterile 4 mM HCl, or simply roll ProDot™ directly into cell culture medium for immediate use. | |
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain |
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