R&D Systems™ ProDots Human IFN-gamma Recombinant Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Manufacturer:  R&D Systems™ PRD28550

Catalog No. PRD28550

Description

The ProDots Recombinant Human IFN-gamma Protein is derived from E. coli. The ProDots Recombinant Human IFN-gamma Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: CAA31639
• Gene ID (Entrez): 3458
• Purity: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Source: E. coli-derived human IFN-gamma protein Gln24-Gln166
• Endotoxin Concentration: <0.10 EU per 1μg of the protein by the LAL method.
• Common Name: IFN-gamma
• Biological Activity: Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is typically 0.15-0.75ng/mL.
• Host Species: Human
• Reconstitution: For a stock solution, reconstitute at 200μg/mL in sterile deionized water, or simply roll ProDot directly into cell culture medium for immediate use.

• For Use With (Application): Bioactivity
• Molecular Weight: Predicted MW: 16.9kDa Observed MW: 17kDa, reducing conditions
• Quantity: 250μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
• Gene Alias: IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma
• Gene Symbol: IFNG
• Conjugate: Unconjugated
• Recombinant: Yes
• Expression System: E. coli-derived Gln24 - Gln166