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R&D Systems™ ProDots Human IFN-gamma Recombinant Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Manufacturer:  R&D Systems™ PRD28550

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Catalog No. PRD28550


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Description

Description

The ProDots Recombinant Human IFN-gamma Protein is derived from E. coli. The ProDots Recombinant Human IFN-gamma Protein has been validated for the following applications: Bioactivity.
Specifications

Specifications

CAA31639
3458
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
E. coli-derived human IFN-gamma protein Gln24-Gln166
<0.10 EU per 1μg of the protein by the LAL method.
IFN-gamma
Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is typically 0.15-0.75ng/mL.
Human
For a stock solution, reconstitute at 200μg/mL in sterile deionized water, or simply roll ProDot directly into cell culture medium for immediate use.
Bioactivity
Predicted MW: 16.9kDa Observed MW: 17kDa, reducing conditions
250μg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
IFG, IFI, IFNG, IFNgamma, IFN-gamma, Immune interferon, interferon gamma, interferon, gamma
IFNG
Unconjugated
Yes
E. coli-derived Gln24 - Gln166
SDS
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Product Certifications
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