R&D Systems™ ProDots Human IL-2 Recombinant Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ PRD20201

Catalog No. PRD20201

Description

The ProDots Recombinant Human IL-2 Protein is derived from E. coli. The ProDots Recombinant Human IL-2 Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P60568
• Gene ID (Entrez): 3558
• Quantity: 5μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
• Gene Alias: aldesleukin, IL2, IL-2lymphokine, interleukin 2, interleukin-2, involved in regulation of T-cell clonal expansion, T cell growth factor, T-cell growth factor, TCGF
• Gene Symbol: IL2
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Expression System: E. coli-derived Ala21 - Thr153, with an N-terminal Met

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): Observed M.W.: 13kDa, reducing conditions, Theoretical M.W.: 15kDa
• Source: E. coli-derived human IL-2 protein Ala21-Thr153,with an N-terminal Met
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Common Name: IL-2
• Biological Activity: Measured in a cell proliferation assay using CTLL-2 mouse cytotoxic T cells. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 295. The ED50 for this effect is typically 0.05-0.25ng/mL
• Species: Human
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile 100 mM Acetic Acid, or simply roll ProDot™ directly into cell culture medium for immediate use.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain