R&D Systems™ ProDots Recombinant Human IL-6 Protein

Supplied as ready-to-use 5 ug protein balls

Supplier:  R&D Systems™ PRD20610

Catalog No. PRD20610

Description

Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813.

The ED50 for this effect is 0.2-0.8 ng/mL.

Specifications

• Accession Number: Q75MH2
• Gene ID (Entrez): 3569
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2°C to 8°C as supplied. 1 month at 2°C to 8°C under sterile conditions after reconstitution. 3 months (at -20°C to -80°C under sterile conditions after reconstitution.)
• Gene Alias: B-cell differentiation factor, B-cell stimulatory factor 2, BSF2, BSF-2, CDF, CTL differentiation factor, HSF, hybridoma growth factor, IFNB2, IFN-beta-2, IL6, IL-6, Interferon beta-2, interleukin 6 (interferon, beta 2), interleukin BSF-2, interleukin-6, MGI-2A
• Gene Symbol: IL6
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Expression System: E. coli-derived Pro29 - Met212
• Protein: IL-6

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): M.W. (Observed): 21 kDa, reducing conditions; M.W. (theoretical): 20.3 kDa
• Source: E. coli-derived human IL-6 protein Pro29 - Met212
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Common Name: IL-6
• Biological Activity: Measured in a cell proliferation assay using T1165.85.2.1 mouse plasmacytoma cells. Nordan, R.P. et al. (1987) J. Immunol. 139:813. The ED50 for this effect is typically 0.2-0.8ng/mL.
• Species: Human
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot directly into cell culture medium for immediate use.
• Purity or Quality Grade: >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.