R&D Systems™ ProDots Human LIF Recombinant Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ PRD773410

Catalog No. PRD773410

Description

The ProDots Recombinant Human LIF Protein is derived from E. coli. The ProDots Recombinant Human LIF Protein has been validated for the following applications: Bioactivity.

Specifications

• Accession Number: P15018
• Gene ID (Entrez): 3976
• Quantity: 50μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
• Gene Alias: CDF, D factor, DIA, differentiation inhibitory activity, differentiation stimulating factor, Differentiation-stimulating factor, Emfilermin, HILDA, HILDAcholinergic differentiation factor, leukemia inhibitory factor, leukemia inhibitory factor (cholinergic differentiation factor), Melanoma-derived LPL inhibitor, MLPLI
• Gene Symbol: LIF
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Expression System: E. coli-derived Pro24 - Phe202

• For Use With (Application): Bioactivity
• Molecular Weight (g/mol): Observed M.W.: 115-137 kDa, reducing conditions; Theoretical M.W.: 19.6 kDa
• Source: E. coli-derived human LIF protein Pro24-Phe202
• Endotoxin Concentration: <0.01 EU per 1μg of the protein by the LAL method.
• Common Name: LIF
• Biological Activity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
• Species: Human
• Reconstitution: For a stock solution, reconstitute at 100 μg/mL in sterile PBS, or simply roll ProDot™ directly into cell culture medium for immediate use.