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R&D Systems™ ProDots Human PDGF-BB Recombinant Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Manufacturer:  R&D Systems™ PRD22050

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Catalog No. PRD22050


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Description

Description

The ProDots Recombinant Human PDGF-BB Protein is derived from E. coli. The ProDots Recombinant Human PDGF-BB Protein has been validated for the following applications: Bioactivity.
Specifications

Specifications

Q6FHE7
5155
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
E. coli-derived human PDGF-BB protein Ser82-Thr190
<0.10 EU per 1μg of the protein by the LAL method.
PDGFB
Unconjugated
Yes
E. coli-derived Ser82 - Thr190
Bioactivity
Observed M.W.: 12kDa, reducing conditions, Theoretical M.W.: 12.3kDa (monomer)
250μg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt at room temperature as supplied. 12 months from date of receipt at 2-8° C as supplied. 1 month at 2-8° C under sterile conditions after reconstitution. 3 months at -20 to -80° C under sterile conditions after reconstitution.
PDGF-BB
Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED50 for this effect is typically 1.5-6.0ng/mL.
Human
For a stock solution, reconstitute at 100 μg/mL in sterile 4 mM HCl, or simply roll ProDot™ directly into cell culture medium for immediate use.
SDS
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Product Certifications
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