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Promega AMV Reverse Transcriptase

AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.

Supplier:  Promega M5101

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Catalog No. PR-M5101

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Includes: 5X Reaction Buffer (250mM Tris-HCl [pH 8.3 at 25°C], 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT)



  • Efficient Reverse Transcription of Templates with High Secondary Structure
  • DNA polymerization using template DNA, RNA or RNA:DNA hybrids
  • Requires a primer as well as magnesium or manganese
  • Stable at higher reaction temperatures (37-58°C)
  • Supplied at a concentration of 10u/µL (Cat.# M5101, M5108) or 20-25u/µL (Cat.# M9004)


First and second strand synthesis of cDNA, primer extensions and RNA sequencing
AMV Reverse Transcriptase
−30 to −10°C
Avian Myeloblastosis Virus
Potassium Phosphate (pH 7.2 at °C) 200mM, 0.2% Triton™ X-100, DTT 2mM and Glycerol 50%
1 x 500μL 5X reaction buffer


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