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Promega AMV Reverse Transcriptase
AMV RT catalyzes DNA polymerization using template DNA, RNA or RNA:DNA hybrids.
Supplier: Promega M9004
Includes: 5X Reaction Buffer (250mM Tris-HCl [pH 8.3 at 25°C], 250mM KCl, 50mM MgCl2, 2.5mM spermidine, 50mM DTT)
Description
- Efficient Reverse Transcription of Templates with High Secondary Structure
- DNA polymerization using template DNA, RNA or RNA:DNA hybrids
- Requires a primer as well as magnesium or manganese
- Stable at higher reaction temperatures (37-58°C)
- Supplied at a concentration of 10u/μL (Cat.# M5101, M5108) or 20-25u/μL (Cat.# M9004)
Specifications
| −30 to −10°C | |
| Liquid | |
| cDNA Synthesis | |
| 1 x 500μL 5X reaction buffer | |
| Potassium Phosphate (pH 7.2 at °C) 200mM, 0.2% Triton™ X-100, DTT 2mM and Glycerol 50% |
| AMV Reverse Transcriptase (HC) | |
| 600U | |
| 20 to 25U/μL | |
| Avian Myeloblastosis Virus |
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