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Promega DNA-Dependent Protein Kinase

Phosphorylates several DNA-binding substrates in vitro, including the tumor suppressor protein p53, the SV40 large T-antigen and several transcription factors

$364.00


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Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
PR-V5811
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Promega
V5811
2,500U
Each for $364.00
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PR-ZV5811
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Promega
ZV5811
This item has been discontinued by the manufacturer and is no longer available. Please call customer service for assistance: 1-800-766-7000.
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Description

Description

  • Isolated from HeLa nuclear extracts as a complex consisting of a 400kDa catalytic subunit and a 155kDa heterodimeric DNA-binding component named Ku, which itself consists of subunits of approximately 85kDa and 70kDa
  • Unit Definition: One unit is defined as the amount of enzyme required to transfer 1pmol of phosphate to DNA-Dependent Protein Kinase Peptide Substrate in 1 minute at 30°C
  • Storage Buffer: 25mM HEPES (pH 7.5), 50mM KCl, 0.2mM EDTA, 10mM MgCl2, 1mM DTT, 10% glycerol

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