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Promega Dual-Luciferase™ Reporter (DLR™) Assay Systems

The Dual-Luciferase Reporter (DLR) Assay System provides an efficient means of performing two reporter assays. In the DLR Assay, the activities of firefly and Renilla luciferases are measured sequentially from a single sample.

Manufacturer:  Promega E1910

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Catalog No. PR-E1910


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Includes: Luciferase Assay Buffer II; Luciferase Assay Substrate (lyophilized product); Stop & Glo Buffer; Passive Lysis 5X Buffer; Stop & Glo Reagent Bottle (E1980 only)

Description

Description

  • Detect firefly and Renilla Luciferase Activities in a Single Sample
  • Renilla luciferase internal control provides more accurate results
  • Range extends over 7 logs; very active samples typically do not need dilution
  • Requires luminometer with 2 injectors

U.S. Pat. Nos. 7,078,181, 7,108,996 and 7,118,878 have been issued to Promega Corporation for a method for increasing luminescence assay sensitivity. Other patents are pending.
This product does not convey a license to use recombinant italics (begin)Renillaitalics (end) luciferase under U.S. Pat. No. 5,418,155 and related patents. Promega sells licensed italics (begin)Renillaitalics (end) luciferase vectors, which may be used in conjunction with this product.
The method of recombinant expression of italics (begin)Coleopteraitalics (end) luciferase is covered by U.S. Pat. Nos. 5,583,024, 5,674,713 and 5,700,673.
U.S. Pat. No. 5,744,320, Australian Pat. No. 721172, European Pat. No. 0 833 939, Canadian Pat. No. 2,221,522, Japanese Pat. No. 3601606 and Korean Pat. No. 10-438880 have been issued to Promega Corporation for quenching reagents and assays for enzyme-mediated luminescence.
U.S. Pat. Nos. 5,641,641, 5,650,289 and 5,814,471, Australian Pat. No. 649289, European Pat. No. 0 553 234 and Japanese Pat. No. 3171595 have been issued to Promega Corporation for a beetle luciferase assay method, which affords greater light output with improved kinetics as compared to the conventional assay.
Certain applications of this product may require licenses from others.

Specifications

Specifications

100 Assays
Dual-Luciferase Reporter Assay System
Luciferase Assay Buffer II; Luciferase Assay Substrate (lyophilized); Stop & Glo Buffer; Passive Lysis 5X Buffer
Safety and Handling

Safety and Handling

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