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Promega PureYield™ Plasmid Midiprep System

PureYield Plasmid Midiprep System provides a fast method for isolating transfection-quality plasmid DNA. Yields 100-200ug of plasmid DNA from 50ml bacteria culture.

Supplier:  Promega A2492

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Catalog No. PR-A2492

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Includes: Midiprep Systems (Mfr. Nos. A2492, A2495, A2496) include cell lysis solution, cell resuspension solution, column wash, endotoxin removal wash, neutralization solution, nuclease-free water, PureYield binding columns, PureYield clearing columns. Start-Up Kit (Mfr. No. A6740) also includes Welch™ Vacuum Pump, 110V; Vac-Man™ Laboratory Vacuum Manifold, 20-sample capacity; and four Eluator™ Vacuum Elution Devices.



  • Up to 200µg of Transfection-Ready Plasmid DNA from 50mL Cultures
  • 30-minute, vacuum-based protocol
  • Highly pure, concentrated plasmid DNA
  • No tedious, gravity-drip columns or post-elution alcohol precipitation


15°C to 30°C
Isolates high-quality plasmid DNA from bacterial cultures
25 Preps
Spin or Vacuum
75mL Cell resuspension solution, 75mL Cell lysis solution, 130mL Neutralization solution, 85.3mL Endotoxin removal wash, 210mL Column wash, 25mL Nuclease-free water, 25 PureYield binding columns, 25 PureYield clearing columns
Plasmid Midiprep System
100 to 200μg


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U.S. Patent 6,194,562, Australian Patent 740145, Canadian Patent 2,329,067.