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Promega PureYield™ Plasmid Midiprep System

PureYield Plasmid Midiprep System provides a fast method for isolating transfection-quality plasmid DNA. Yields 100-200ug of plasmid DNA from 50ml bacteria culture.

Supplier:  Promega A2495

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Catalog No. PRA2495

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Includes: Midiprep Systems (Mfr. Nos. A2492, A2495, A2496) include cell lysis solution, cell resuspension solution, column wash, endotoxin removal wash, neutralization solution, nuclease-free water, PureYield binding columns, PureYield clearing columns. Start-Up Kit (Mfr. No. A6740) also includes Welch™ Vacuum Pump, 110V; Vac-Man™ Laboratory Vacuum Manifold, 20-sample capacity; and four Eluator™ Vacuum Elution Devices.



  • Up to 200µg of Transfection-Ready Plasmid DNA from 50mL Cultures
  • 30-minute, vacuum-based protocol
  • Highly pure, concentrated plasmid DNA
  • No tedious, gravity-drip columns or post-elution alcohol precipitation


15°C to 30°C
Isolates high-quality plasmid DNA from bacterial cultures
100 Preps
Spin or Vacuum
315mL Cell resuspension solution, 315mL Cell lysis solution, 500mL Neutralization solution, 315mL Endotoxin removal wash, 2 × 381mL Column wash, 4 × 25mL Nuclease-free water, 100 PureYield binding columns, 100 PureYield clearing columns
Plasmid Midiprep System
100 to 200μg


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U.S. Patent 6,194,562, Australian Patent 740145, Canadian Patent 2,329,067.