Promega PureYield™ Plasmid Midiprep System
PureYield Plasmid Midiprep System provides a fast method for isolating transfection-quality plasmid DNA. Yields 100-200ug of plasmid DNA from 50ml bacteria culture.
Supplier: Promega A2495
- Up to 200µg of Transfection-Ready Plasmid DNA from 50mL Cultures
- 30-minute, vacuum-based protocol
- Highly pure, concentrated plasmid DNA
- No tedious, gravity-drip columns or post-elution alcohol precipitation
|15°C to 30°C|
|Isolates high-quality plasmid DNA from bacterial cultures|
|Spin or Vacuum|
|315mL Cell resuspension solution, 315mL Cell lysis solution, 500mL Neutralization solution, 315mL Endotoxin removal wash, 2 × 381mL Column wash, 4 × 25mL Nuclease-free water, 100 PureYield binding columns, 100 PureYield clearing columns|
|Plasmid Midiprep System|
|100 to 200μg|
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U.S. Patent 6,194,562, Australian Patent 740145, Canadian Patent 2,329,067.