Promega PureYield™ Plasmid Midiprep System

PureYield Plasmid Midiprep System provides a fast method for isolating transfection-quality plasmid DNA. Yields 100-200ug of plasmid DNA from 50ml bacteria culture.

Includes: Midiprep Systems (Mfr. Nos. A2492, A2495, A2496) include cell lysis solution, cell resuspension solution, column wash, endotoxin removal wash, neutralization solution, nuclease-free water, PureYield binding columns, PureYield clearing columns. Start-Up Kit (Mfr. No. A6740) also includes Welch™ Vacuum Pump, 110V; Vac-Man™ Laboratory Vacuum Manifold, 20-sample capacity; and four Eluator™ Vacuum Elution Devices.

Catalog Number	Mfr. No.	Includes	No. of Reactions
PRA6740	Promega A6740	Midiprep System (100 preps), Vac-Man Laboratory Vacuum Manifold (20-sample capacity), 4 Eluator elution devices, Vacuum pump	100 preps
PR-A2492	Promega A2492	75mL Cell resuspension solution, 75mL Cell lysis solution, 130mL Neutralization solution, 85.3mL Endotoxin removal wash, 210mL Column wash, 25mL Nuclease-free water, 25 PureYield binding columns, 25 PureYield clearing columns	25 Preps
PRA2495	Promega A2495	315mL Cell resuspension solution, 315mL Cell lysis solution, 500mL Neutralization solution, 315mL Endotoxin removal wash, 2 × 381mL Column wash, 4 × 25mL Nuclease-free water, 100 PureYield binding columns, 100 PureYield clearing columns	100 Preps
PRA2496	Promega A2496	3 × 315mL Cell resuspension solution, 3 × 315mL Cell lysis solution, 3 × 500mL Neutralization solution, 3 × 315mL Endotoxin removal wash, 6 × 381mL Column wash, 2 × 150mL Nuclease-free water, 300 PureYield binding columns, 300 PureYield clearing columns	300 Preps

Description

• Up to 200µg of Transfection-Ready Plasmid DNA from 50mL Cultures
• 30-minute, vacuum-based protocol
• Highly pure, concentrated plasmid DNA
• No tedious, gravity-drip columns or post-elution alcohol precipitation

Specifications

• Content And Storage: 15°C to 30°C
• For Use With (Application): Isolates high-quality plasmid DNA from bacterial cultures
• Yield: 100 to 200μg

• Format: Spin or Vacuum
• Sample Volume: 50mL

U.S. Patent 6,194,562, Australian Patent 740145, Canadian Patent 2,329,067.