Promega Restriction Enzymes - Sgf I Genome Qualified (C)
Genome qualified to ensure optimal performance in genomic analysis applications. Blue/white cloning qualified, providing a higher level of quality control for enzymes used in cloning applications.
- Star activity may be observed with glycerol concentrations >12% or with enzyme:DNA ratios >25u/µg
- Sensitive to CpG methylation
- SgfI is inactivated by freezing at -70°C
- Ends generated by SgfI can be directly ligated to ends generated by PvuI. The PvuI site is regenerated in the ligation product, but the SgfI site is not
Restriction enzyme buffer included indicated in parentheses; Cloning = Blue/White Cloning Qualified; Genome = Genome Qualified.
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