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Promega SP6 RNA Polymerase

DNA-dependent RNA polymerase for in vitro transcription.

Manufacturer:  Promega P4084

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Catalog No. PR-P4084

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  • DNA-Dependent RNA Polymerase Specific for SP6 Promoter Sequences
  • Exhibits extremely high affinity and specificity for SP6 promoter sequences
  • >90% pure as determined by SDS polyacrylamide gel electrophoresis
  • Free of detectable levels of contaminating RNase and DNase activity (


SP6 RNA Polymerase
100mM DTT 5X Reaction Buffer, transcription optimized 5X Buffer: 200mM Tris HCl (pH 7.9 at 25°C), 30mM MgCl2, 10mM Spermidine, 50mM NaCl
20mM Potassium Phosphate (pH 7.7 at 25°C), 1mM EDTA, 10mM DTT, 100mM NaCl, 0.1% Triton™ X-100, 50% Glycerol
Recombinant E. coli strain
Generation of templates for in-vitro translation, generation of probes for Nucleic Acid hybridization, generation of RNA processing Substrates, generation of Antisense RNA
−30 to −10°C


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