Thermo Scientific™ ProPac™ HIC-10 HPLC Columns

Catalog Number	Mfr. No.	Particle Size	Diameter (Metric)	Length (Metric)
DX063653	Thermo Scientific™ 063653	5 μm	2.1 mm	100 mm
DX063655	Thermo Scientific™ 063655	5 μm	4.6 mm	100 mm
DX063665	Thermo Scientific™ 063665	5 μm	7.8 mm	75 mm
DX074197	Thermo Scientific™ 074197	5 μm	4.6 mm	250 mm

Description

Achieve high-resolution separation of proteins and peptides under nondenaturing conditions with Thermo Scientific™ ProPac™ HIC-10 LC Columns. Developed with multi-functional ligand attachments, these silica-based columns provide exceptional hydrolytic stability under the highly aqueous conditions used in HIC.

High-efficiency Columns for the Separation of Large Biomolecules

• High protein loading capacity for protein purification applications
• Wide range of analytical and preparative applications, including the separation of bovine serum proteins, snake venom proteins, and monoclonal antibodies (Mabs)
• Based on 5μm ultra-high purity spherical silica gel particles with 300Å pores

Hydrophobic interaction chromatography separates biomolecules in a decreasing salt gradient, based on differences in surface hydrophobicity. This method preserves the biological activity of proteins. The HIC separation mechanism is complementary to those of ion-exchange and gel filtration chromatography.

Specifications

• Max. Pressure: 3500 psi (240 bar)
• pH: 2.5 to 7.5
• Surface Area: 100 m²/g
• Stationary Phase: Amide/Ethyl
• Column Type: Hydrophobic Interaction
• Material: Stainless Steel

• Particle Size: 5 μm
• Pore Size: 300 Å
• Temperature: 40°C
• Packing Material: Spherical, Fully Porous, Ultrapure Silica
• Phase: Hydrophobic Interaction

Limited warranty period: Subject to all warranty requirements and exclusions set out in our Terms and Conditions of Sale, this product is warranted from the date we ship the product and for ninety (90) days thereafter. Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.