Thermo Scientific™ ProSwift™ RP-4H Capillary Monolithic HPLC Columns

For fast, high-resolution LC-MS separations in protein identification, biomarker discovery, and systems biology applications, use Thermo Scientific™ ProSwift™ RP-4H Capillary Monolithic HPLC Columns.

Manufacturer: Thermo Scientific™ 164923

 View more versions of this product

Catalog No. 16-492-3

  Check Availability
Add to Cart


    Column Format Analytical Column
    Diameter (Metric) 0.2mm
    Packing Material Monolithic polymer
    Stationary Phase Phenyl
    Length (Metric) 250mm
    USP Type N/A
    Column Type Protein
    Flow Rate 2 to 12µL/min
    Max. Pressure 4950psi
    Particle Size Monolith
    Temperature 70°C
    Pore Size -
    pH 1 to 14
    For Use With LC-MS
    Material Poly(ethylvinylbenzene-co-divinylbenzene)
    Solvents Most common Organic Solvents
    Surface Area -
    Type Analytical Column
    Carbon Load -
    Description ProSwift RP-4H, medium channel
    Column Chemistry Phenyl
    Diameter 200µm ID
    Endcapped -

    For fast, high-resolution LC-MS separations in protein identification, biomarker discovery, and systems biology applications, use Thermo Scientific™ ProSwift™ Monolithic HPLC Columns. Based on a ethylvinylbenzene divinylbenzene copolymer, the monolithic structure offers a high-quality alternative to traditional microparticulate sorbents, providing important advantages to the chromatographic separation. High-sensitivity proteomics and biotech applications are easily performed using these columns.

    Each monolith is a single cylindrical polymer rod containing an uninterrupted, interconnected network of flow-through channels of a specific pore size. These large channels and the monolith's nonporous surfaces provide fast mass transfer for the high-resolution and fast protein separations. The monolith morphology results in low operating backpressure, allowing the use of higher linear velocities with minimal loss of resolution.

    Advantages of High Resolution at Exceptionally High Flow Rates

    • Highest operational flow rates available
    • High throughput and improved productivity
    • Excellent stability over a wide pH range of 1 to 14
    • Outstanding reproducibility and ruggedness
    • High loading capacity
    Fast, Sensitive Protein Separations
    • High speed protein separations
    • High sensitivity for LC-MS
    • High column-to-column reproducibility
    • Wide range of column I.D. and lengths available
    • Superior lifetime
    Easy Column Installation
    • Use Thermo Scientific™ Dionex™ nanoViper™ Fingertight Fittings
    Preconcentrate and Desalt Samples   
    • Precolumns are available to preconcentrate and desalt samples.
    • You can also use Thermo Scientific™ PepSwift™ Trap Columns for preconcentration and desalting of samples consisting of peptides and proteins without negatively impacting the chromatographic performance or recovery of the compounds.
    • Change the selectivity of the separation or improve the trapping efficiency by employing various ion-pairing agents in the loading solvent and/or mobile phases.

  • Description & Specifications

Related Products

Product Content Feedback

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit