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BioVision IncSupplier Diversity Partner Proteasome Activity Fluorometric Assay Kit: Proteasome Activity Assay Kit: Detect Proteasome Activity within 30-60 min. Simple, Reliable & Sensitive. Detection Method: Fluorescence. 100 Assays. DFS Item

Manufacturer:  BioVision IncSupplier Diversity Partner K245100

Proteasome Activity Fluorometric Assay Kit: Proteasomes are very large (20S, 26S) protein assemblies found in both the nucleus and cytoplasm of all eucaryotes (and in some procaryotes) which are responsible for the degradation and recycling of proteins which have been previously tagged with ubiquitin. Such tagged proteins are degraded into peptides approximately 7-8 amino acids long which are subsequently further degraded. BioVision's Proteasome Activity Assay takes advantage of the chymotrypsin-like activity, utilizing an AMC-tagged peptide substrate which releases free, highly fluorescent AMC in the presence of proteolytic activity. The kit also includes a positive control (Jurkat Cell lysate with significant proteasome activity) and a specific proteasome inhibitor MG-132 which suppresses all proteolytic activity due to proteasomes, which make the assay specifically measures proteasome activity from other protease activity which may be present in samples.

Catalog No. 50-111-9643


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