MilliporeSigma™ Proteinase K Solution, 600mAU/mL

Efficient removal of proteins from nucleic acid solutions

Catalog Number	Mfr. No.	Quantity
71-049-4	MilliporeSigma™ 710494	10 mL
71-049-3	MilliporeSigma™ 710493	2 mL

Description

• Proteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album
• The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA
• Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C)
• The recommended working concentration is 50-100μg/mL for protein removal and enzyme inactivation, and up to 2mg/mL for tissue treatment
• The Proteinase K, Lyophilized powder can be prepared as a 20mg/mL stock solution in water and stored in aliquots at -20°C
• The enzyme is also available as a ready-to-use concentrated stock solution (600mAU/mL) that is convenient for routine use in most applications
• 1mg of Proteinase K is the equivalent of 30mAU (AU = Anson unit)
• The Novagen Proteinase K products are free of detectable DNase and RNase

Specifications

• Content And Storage: -20°C
• Product Type: Proteinase K