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Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit

The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes.

Supplier:  Invitrogen™ A35892


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Catalog No. A35892

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The simple precipitation-free protocol yields up to 0.4mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.
  • Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
  • Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/µg endotoxins
  • High yields—isolate up to 0.4 mg of high-quality plasmid DNA
  • Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization


• Resuspension buffer, store at 4°C upon reciept
• Lysis buffer, store at room temperature
• Precipitation buffer, store at room temperature
• Binding buffer, store at room temperature
• Wash buffer 1, store at room temperature
• Wash buffer 2 (concentrate), store at room temperature
• Elution buffer, store at room temperature
• Column assemblies, store at room temperature
• Syringe filters, store at room temperature
• Syringe plungers, store at room temperature
• Collection tubes, store at room temperature

Silica Column
Cloning, PCR
Midi scale input culture of 25-100 mL to obtain yields of up to 200 µg
Plasmid Purification Kit
Room Temperature
25 Preps
25 Preps
30 min


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