Invitrogen™ PureLink™ Fast Low-Endotoxin Midi Plasmid Purification Kit

The PureLink Fast Low-Endotoxin Midi Plasmid Purification Kit uses next-generation columns with advanced silica membranes that enable isolation of transfection grade (<1 EU/µg) plasmid DNA in about 30 minutes.

Supplier:  Invitrogen™ A36227

Catalog No. A36227

Description

The simple precipitation-free protocol yields up to 0.4mg plasmid, suitable for standard transfections and all molecular biology applications such as cloning and sequencing.

• Ultra-fast protocol—innovative silica-based columns enable purification of transfection grade plasmid DNA in under 30 minutes
• Low endotoxin plasmid—the resulting plasmid contains less than 1 EU/µg endotoxins
• High yields—isolate up to 0.4 mg of high-quality plasmid DNA
• Colored buffers—the kit features colored buffers that permit error-free visualization of complete bacterial cell lysis and subsequent neutralization

Specifications

• Content And Storage: • Resuspension buffer, store at 4°C upon reciept
  • Lysis buffer, store at room temperature
  • Precipitation buffer, store at room temperature
  • Binding buffer, store at room temperature
  • Wash buffer 1, store at room temperature
  • Wash buffer 2 (concentrate), store at room temperature
  • Elution buffer, store at room temperature
  • Column assemblies, store at room temperature
  • Syringe filters, store at room temperature
  • Syringe plungers, store at room temperature
  • Collection tubes, store at room temperature
  
  
• Isolation Technology: Silica Column
• For Use With (Application): Cloning, PCR
• Prep Scale: Midi scale input culture of 25-100 mL to obtain yields of up to 200 µg
• Product Type: Plasmid Purification Kit
• Shipping Condition: Room Temperature

• Format: Kit
• Scale: Midi
• No. of Reactions: 50 Preps
• Product Line: PureLink™
• Quantity: 50 Preps
• Test Time: 30 min