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Thermo Scientific™ PvuII (10 U/μL)

The PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer.

Supplier:  Thermo Scientific™ ER0631

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Catalog No. FERER0631



Description

Description

Lambda DNA digested with PvuII, 0.7% agarose, 15 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'  C  A  G ↓C  T  G   3' 
3'  G  T  C ↑G  A  C   5' 

Thermo Scientific PvuII restriction enzyme recognizes CAG^CTG sites and cuts best at 37°C in G buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity, refer to product specifications.
TRUSTED_SUSTAINABILITY
Specifications

Specifications

10 U/μL
Pvu II
No
No
Traditional Cloning
Not Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
10X Buffer G
37°C
Restriction Enzyme
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For Research Use Only. Not for use in diagnostic procedures.