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R&D Systems™ CometAssay Single Cell Gel Electrophoresis Assay-Silver
Provides a simple and effective method for evaluating DNA damage in cells
Supplier: R&D Systems™ 4250050K
Includes: Lysis Solution, 2 x 500mL; Comet LMAgarose, 15mL; Trevigen CometSlide, 25/kit; pH 10 200mM EDTA, 12.5mL; Kit with Silver Stain (Mfr. No. 4251-050-K) also includes four staining reagents and a fixation additive.
Description
The principle of the assay is based upon the ability of denatured, cleaved DNA fragments to migrate out of the nucleoid under the influence of an electric field, whereas undamaged DNA migrates slower and remains within the confines of the nucleoid when a
Choose the Neutral CometAssay to detect double-stranded breaks or the Alkaline CometAssay for a more sensitive protocol used to detect smaller amounts of damage including single and double-stranded breaks
Cells are immobilized in a bed of low melting poi
Specifications
| 50 Tests | |
| Lysis Solution (2 x 500mL), Comet LMAgarose (15mL); Trevigen CometSlide (25/kit) and pH 10 200mM EDTA (12.5mL) | |
| Single Cell Gel Electrophoresis Assay | |
| The product requires storage at both 2 - 8° C and room temperature. Consult the product insert for specific storage temperatures. Do not use past expiration date. |
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