R&D Systems E. coli Endonuclease III/Buffer

Purified from E. coli containing a recombinant plasmid harboring the E. coli nth gene

Manufacturer: R&D Systems 4045-01K-EB

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Catalog No. 404501KEB

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    Product Name E. coli Endonuclease III/Buffer
    Quantity 1000U

    Endonucleases are enzymes that cleave nucleic acids (RNA or DNA) within the nucleotide strand. Endonuclease III is a DNA glycosylase with an associated AP lyase activity. Endonuclease III releases bases damaged by UV light, ionizing radiation, osmium tetroxide or acid. It cleaves abasic sites by b-elimination, producing a single nucleotide gap in the DNA, and contains an iron-sulfur group which helps to maintain its three dimensional conformation.

    • One Unit of enzyme cleaves 1 pmole of an oligonucleotide probe containing an AP site within an oligonucleotide duplex in one hour at 37°C
    • Catalyzes the excision of the following forms of DNA damage: Cis-trans-thymine glycol, 5,6-dihydrothymine, 5,6-dihydroxydihydrothymine, alloxan, urea, uracil, 5-hydroxy-5-methylhydantoin, methyltartronylurea, 6-hydroxy-5,6-dihydropyrimidines, 5-hydroxycytosine, 5-hydroxyuracil, 5-hydroxy-6-hydrothymine, 5,6-dihydrouracil, glycol, 5-hydroxy-6-hydrouracil, and AP sites
    • Suitable for use in FLARE™

  • Description & Specifications

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