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R&D Systems HT Homogeneous PARG Chemiluminescent Inhibition Assay
A highly sensitive high-throughput chemiluminescence screening assay for PARG inhibitors in vitro
Manufacturer: R&D Systems 4691096K
Includes: 100mU/uL, 50uL; 10x PARG Dilution Buffer, 1mL; D-Luciferin, 900uL; Luciferase Cycling Enzymes, 600uL; 10x dCTP, 900uL; PARG Substrate, 600uL; Cycling Buffer, 6mL; 96-Well White Round Bottom Plate
Description
Poly(ADP-ribose) glycohydrolase (PARG) is a DNA repair protein which regulates the length of poly(ADP-ribose) (PAR) polymers synthesized by poly(ADP-ribose) polymerase (PARP) during the repair of DNA single-strand
breaks. Knockdown studies have shown that
This end point assay is performed in one step for rapid, easy use
Luciferase cleaves ATP into AMP in the presence of the substrate luciferin, generating a quantifiable light signal
AMP reenters the cycling reaction assay to sustain the light signal for
Specifications
96 Tests |
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