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R&D Systems™ Recombinant Human ACE-2 Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Enzyme Activity
Supplier: R&D Systems™ 933ZN010
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Description
Measured by its binding ability in a functional ELISA. When Recombinant SARS-CoV-2 Spike RBD Fc Chimera (Catalog # 10499-CV ) is immobilized at 0.2 μg/mL (100 μL/well), Recombinant Human ACE-2 (Catalog # 933-ZN) binds with an ED50 of 1.00-12.0 ng/mL. Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is >800 pmol/min/μg, as measured under the described conditions.Specifications
Q9BYF1 | |
Supplied as a 0.2 μm filtered solution in Tris, NaCl, ZnCl2 and Glycerol. | |
M.W. (Observed): 101-111 kDa, reducing conditions; M.W. (theoretical): 85 kDa | |
10 μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening. | |
<1.0 EU / 1 μg of the protein by the LAL method. | |
Unconjugated | |
Measured by its binding ability in a functional ELISA with Recombinant Viral SARS-CoV-2 S Protein RBD Fc Chimera (Catalog # 10499-CV). Measured by its ability to cleave a fluorogenic peptide substrate, Mca-YVADAPK(Dnp)-OH (Catalog # ES007 ). The specific activity is >800 pmol/min/μg, as measured under the described conditions. | |
ACE-2 |
Enzyme Activity | |
59272 | |
ACE-2 | |
Mouse myeloma cell line, NS0-derived human ACE-2 protein Gln18-Ser740, with a C-terminal 10-His tag | |
Recombinant Human ACE-2 is prone to proteolytic cleavage at C-terminus. The predominant form of the purified protein lacks the His tag. | |
ACE2 | |
Recombinant | |
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain. |
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