R&D Systems™ Recombinant Human B7-2/CD86 Fc Chimera Protein

A New rh B7-2 is Available! It has higher purity, ∼3 fold better activity, and is HEK293 expressed!

Supplier:  R&D Systems™ 141B2100

Catalog No. 141B2100

Description

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.2-0.8 μg/mL in the presence of PHA.

Specifications

• Accession Number: AAB03814
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
• Molecular Weight (g/mol): M.W. (Observed): 85-95 kDa, reducing conditions; M.W. (theoretical): 52.7 kDa (monomer)
• Quantity: 100 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Protein: B7-2/CD86

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 942
• Name: B7-2/CD86 Fc Chimera
• Source: Mouse myeloma cell line, NS0-derived human B7-2/CD86 protein Human B7-2 (Leu20-His239) (N-terminus) Accession # AAB03814 DIEGRMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
• Structural Form: Disulfide-linked homodimer
• Gene Symbol: CD86
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain