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R&D Systems™ Recombinant Human B7-H3 (4Ig)/B7-H3b Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 2318B3050

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Catalog No. 2318B3050


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Description

Description

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated human T cells. T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well plates coated with 500 ng/mL anti-CD3 and/or 10 μg/mL rhB7-H3 (4Ig)/B7-H3b. The presence of rhB7-H3 (4Ig)/B7-H3b at 10 μg/mL inhibited the anti-CD3 response by >30%. Optimal dilutions should be determined by each laboratory for each application.
Specifications

Specifications

Q5ZPR3
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
M.W. (Observed): 78-90 kDa, reducing conditions; M.W. (theoretical): 48 kDa
50 μg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
CD276
Recombinant
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Bioactivity
80381
B7-H3 (4Ig)/B7-H3b
Mouse myeloma cell line, NS0-derived human B7-H3 protein Gly27-Thr461, with a C-terminal 10-His tag
<0.10 EU / 1 μg of the protein by the LAL method.
Unconjugated
Reconstitute at 100 μg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
B7-H3/CD276
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