R&D Systems™ Recombinant Human Chitinase 3-like 1 Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 2599CH050

Catalog No. 2599CH050

Description

Measured by the ability of the immobilized protein to support the adhesion of FaDu human squamous cell carcinoma cells. When 5 x 104 cells per well are added to recombinant human CHI3-L1 coated plate, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.6-2.4 μg/mL.

Specifications

• Accession Number: P36222
• Formulation: Lyophilized from a 0.2 µm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 38-41 kDa, reducing conditions; M.W. (theoretical): 41.3 kDa (monomer)
• Quantity: 50 µg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Gene Symbol: CHI3L1
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 1116
• Name: Chitinase 3-like 1
• Source: Mouse myeloma cell line, NS0-derived human Chitinase 3-like 1 protein Tyr22-Thr383, with a C-terminal 6-His tag
• Endotoxin Concentration: <0.10 EU / 1 µg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 200 µg/mL in PBS.
• Protein: Chitinase 3-like 1