DescriptionMeasured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Rubin, J.S. et al. (1991) Proc. Natl. Acad. Sci. USA 88:415. The ED50 for this effect is 20-100 pg/mL. The specific activity of Recombinant Human EGF is approximately 1.4 x 103 IU/μg, which is calibrated against human EGF WHO International Standard (NIBSC code: 91/530).
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|Lyophilized from a 0.2 µm filtered solution in PBS.|
|M.W. (Observed): 6 kDa, reducing conditions; M.W. (theoretical): 6 kDa|
|>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.|
|E. coli-derived human EGF protein Asn971-Arg1023, with an N-terminal Met|
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