R&D Systems Human Endocan/ESM-1 Recombinant Protein
Manufacturer: R&D Systems 1810EC050
DescriptionMeasured by its ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. Bechard, D. et al. (2001) J. Immunol. 167:3099. When 1 x 10 5 cells/well are added to Recombinant Human Endocan/ESM coated plates, adhesion is induced in a dose dependent manner after a 1 hour incubation at 37 degreesC. The ED 50 for this effect is 2-10 µg/mL. Optimal dilutions should be determined by each laboratory for each application.
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|Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.|
|90%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|