R&D Systems™ Recombinant Human ENPP-7/Alk-SMase Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ 4924EN010

Catalog No. 4924EN010

Description

Measured by its ability to hydrolyze sphingomyelin to ceramide and phosphorylcholine. The phosphorylcholine is cleaved by Recombinant Human Alkaline Phosphatase/ALPL (Catalog # 2909-AP ) and the phosphate is detected/measured by a Malachite Green Phosphate Detection Kit (Catalog # DY996 ). The specific activity is >14,000 pmol/min/μg, as measured under the described conditions.

Specifications

• Accession Number: NP_848638
• Formulation: Supplied as a 0.2 μm filtered solution in Tris and NaCl.
• Molecular Weight (g/mol): M.W. (Observed): 60-70 kDa, reducing conditions; M.W. (theoretical): 49 kDa
• Quantity: 10 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
• Gene Symbol: ENPP7
• Recombinant: Recombinant
• Protein: ENPP-7/Alk-SMase

• For Use With (Application): Enzyme Activity
• Gene ID (Entrez): 339221
• Name: ENPP-7/Alk-SMase
• Source: Mouse myeloma cell line, NS0-derived human ENPP-7/Alk-SMase protein Ala22-Ser439, with a C-terminal 10-His tag
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain