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R&D Systems™ Recombinant Human Fucosyltransferase 5/FUT5 Protein

FUT5 has been formulated so that it can be used in the cell surface glycoengineering of living cells, and does not affect cell viability or native phenotype apart from the intended impact on cell glycobiology.

Supplier:  R&D Systems™ 4949GT020

Catalog No. 4949GT020


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Description

Description

Measured by its ability to transfer fucose from GDP-fucose to N -Acetyllactosamine The specific activity is >35 pmol/min/μg, as measured under the described conditions.
Specifications

Specifications

Q11128
Supplied as a 0.2 μm filtered solution in Tris and NaCl.
M.W. (Observed): 58 kDa, reducing conditions; M.W. (theoretical): 40 kDa
20 μg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20°C to -70°C as supplied. 3 months, -20°C to -70°C under sterile conditions after opening.
<1.0 EU / 1 μg of the protein by the LAL method.
Unconjugated
>80%, by SDS-PAGE under reducing conditions and visualized by silver stain
Enzyme Activity
2527
Fucosyltransferase 5/FUT5
Mouse myeloma cell line, NS0-derived human Fucosyltransferase 5/FUT5 protein Arg35-Thr374, with an N-terminal 6-His tag
Disulfide-linked homodimer
FUT5
Recombinant
Fucosyltransferase 5/FUT5
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