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R&D Systems™ Recombinant Human His6-UbcH6/UBE2E1 Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ E2630100

Catalog No. E2630100

Item Discontinued This item has been discontinued by the supplier. Please to view product availability in your area.

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Description

Description

Recombinant Human His6-UbcH6/UBE2E1 is a member of the Ubiquitin-conjugating (E2) enzyme family that receives Ubiquitin from a Ubiquitin-activating (E1) enzyme and subsequently interacts with a Ubiquitin ligase (E3) to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human His6-UbcH6/UBE2E1 concentration of 0.1-1 μM.
Specifications

Specifications

P51965
X mg/mL (X μM) in 50 mM HEPES pH 7.5, 200 mM NaCl, 10% Glycerol (v/v), 1 mM TCEP.
M.W. (theoretical): 23 kDa
100 μg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70°C as supplied. 3 months, -70°C under sterile conditions after opening.
Unconjugated
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie™ Blue stain
Enzyme Activity
7324
His6 UbcH6/UBE2E1
E. coli-derived human UbcH6/UBE2E1 protein Contains an N-terminal Gly-Ser-Ser and 6-His tag
UBE2E1
Recombinant
UbcH6/UBE2E1
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