R&D Systems Human MDM2/HDM2 Ubiquitin Ligase Kit - p53 Substrate
Designed for the in vitro ubiquitination of p53 by MDM2 with subsequent Western blot
Manufacturer: R&D Systems K200B
The RING-finger ubiquitin E3 ligase MDM2 (Murine Double Minute 2) is an important regulator of the tumor suppressor protein p53. MDM2 ligase activity is at least partially responsible for the ubiquitination and subsequent proteasomal degradation of p53.
Ubiquitinated proteins can be used in downstream applications, or analyzed by Western blot using antibodies specific for the target protein
This kit can be used as a positive control in studies that monitor the activities of other known or putative E3 ligase enzymes that ubiquitinate p53
Ubiquitinated p53 substrate can also be used to study E4 ligase activities of enzymes such as UBE4B, or the activity of deubiquitinating enzymes such as USP7
|Store the unopened product at -70°C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.|
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