R&D Systems Human/Mouse/Rat/Canine/Equine BDNF Recombinant Protein
Manufacturer: R&D Systems 248BD025/CF
DescriptionMeasured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED 50 for this effect is 0.2-2 ng/mL. The specific activity of Recombinant Human/Mouse/Rat/Canine/Equine BDNF is approximately 1.3 x 10 3 units/µg, which is calibrated against recombinant human BDNF WHO Standard (NIBSC code: 96/534). Measured by its binding ability in a functional ELISA. When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK ) is coated at 1 µg/mL, Recombinant Human BDNF binds with an apparent K d <1 nM.
|Lyophilized from a 0.2μm filtered solution in Sodium Citrate and NaCl.|
|97%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|
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