R&D Systems™ Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity, Binding Activity
Manufacturer: R&D Systems™ 1054OX010
DescriptionMeasured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. Muller, N. et al. (2008) FEBS J. 275:2296. The ED50 for this effect is typically 1-5 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40/TNFRSF4 Fc Chimera (Catalog # 3388-OX ) is immobilized at 10 ng/mL, the concentration of Recombinant Human OX40 Ligand/TNFSF4 that produces 50% optimal binding response is found to be approximately 0.5-3 ng/mL.
|Bioactivity, Binding Activity|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.|
|Lyophilized from a 0.2 µm filtered solution in PBS.|
|M.W. (Observed): 28-34 kDa, reducing conditions; M.W. (theoretical): 18 kDa|
|>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
|Mouse myeloma cell line, NS0-derived human OX40 Ligand/TNFSF4 protein MHHHHHHHHHH GGGSGGGSGGGS IEGR Human OX40 Ligand (Gln51-Leu183) (N-terminus) Accession # Q6FGS4 (C-terminus)|
|<1.0 EU / 1 µg of the protein by the LAL method.|
|Reconstitute at 100 µg/mL in sterile PBS.|
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