R&D Systems™ Recombinant Human SIRP alpha/CD172a Fc Chimera Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ 4546SA050

Catalog No. 4546SA050

Description

Measured by the ability of the immobilized protein to support the adhesion of human red blood cells. Subramanian, S. et al. (2006) Blood 107:2548. Red blood cells, added at 10 x 106 cells/mL (100 μL/well) to SIRP alpha coated plates, will adhere after 1 hour at 37° C. The ED50 for this effect is 0.6-2.4 μg/mL. Optimal dilutions should be determined by each laboratory for each application.

Specifications

• Accession Number: NP_542970
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 70-105 kDa, reducing conditions; M.W. (theoretical): 64 kDa (monomer)
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Protein: SIRP alpha/CD172a

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 140885
• Name: SIRP alpha/CD172a Fc Chimera
• Source: Chinese Hamster Ovary cell line, CHO-derived human SIRP alpha/CD172a protein Human SIRP alpha (Gly27-Arg370) & (Glu31-Arg370) (N-terminus) Accession # NP_542970 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Structural Form: Disulfide-linked homodimer
• Gene Symbol: SIRPA
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.