R&D Systems™ Recombinant Human TNF-alpha Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 210TA020

Catalog No. 210TA020

Description

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED50 for this effect is 25-100 pg/mL.

Specifications

• Formulation: Lyophilized from a 0.2μm filtered solution in PBS with BSA as a carrier protein.
• Molecular Weight (g/mol): 17.5 kDa
• Quantity: 20μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.
• Conjugate: Unconjugated
• Purity or Quality Grade: 97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Gene ID (Entrez): 7124
• Name: TNF-alpha
• Source: E. coli-derived human TNF-alpha protein Val77-Leu233,with and without an N-terminal Met
• Gene Alias: APC1 protein, Cachectin, Cachetin, DIF, TNF, TNF, monocyte-derived, TNF-a, TNFA, TNFalpha, TNF-alphacachectin, TNFATNF, macrophage-derived, TNFG1F, TNFSF1A, TNFSF2, TNFSF2TNF superfamily, member 2, tumor necrosis factor, tumor necrosis factor (TNF superfamily, member 2), tumor necrosis factor alpha, Tumor necrosis factor ligand superfamily member 2, tumor necrosis factor-alpha
• Recombinant: Recombinant