R&D Systems™ Recombinant Mouse 4-1BB Ligand/TNFSF9 Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity, Binding Activity

Supplier:  R&D Systems™ 12464L025/CF

Catalog No. 12464L025CF

Description

Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 0.008-0.05 μg/mL in the presence of a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ). Measured by its ability to bind Recombinant Mouse 4-1BB/TNFRSF9/CD137 Fc Chimera (Catalog # 937-4B ) in a functional ELISA.

Specifications

• Accession Number: P41274
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 41-44 kDa, reducing conditions; M.W. (theoretical): 25.7 kDa
• Source: Mouse myeloma cell line, NS0-derived mouse 4-1 BB Ligand/TNFSF9 protein Met 10-His tag GGGSGGGSGGGSIEGR Mouse 4-1 BB Ligand (Arg104-Glu309) (N-terminus) Accession # P41274 (C-terminus)
• Endotoxin Concentration: <1.0 EU / 1 μg of the protein by the LAL method.
• Product Type: 4-1BB Ligand/TNFSF9
• Species: Mouse
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Protein: 4-1 BB Ligand/TNFSF9

• For Use With (Application): Bioactivity, Binding Activity
• Gene ID (Entrez): 8744
• Quantity: 25 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Gene Symbol: Tnfsf9
• Conjugate: Unconjugated
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.