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R&D Systems™ Recombinant Mouse CD40/TNFRSF5 Fc Chimera Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Manufacturer:  R&D Systems™ 1215CD050

Catalog No. 1215CD050


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Description

Description

Measured by its binding ability in a functional ELISA. When Recombinant Mouse CD40/TNFRSF5 Fc Chimera is immobilized at 1 μg/mL (100 μL/well), the concentration of Recombinant Mouse CD40 Ligand/TNFSF5 (Catalog # 8230-CL ) that produces 50% optimal binding is found to be 100-600 ng/mL.
Specifications

Specifications

P27512
Lyophilized from a 0.2 µm filtered solution in PBS.
M.W. (Observed): 58-61 kDa, reducing conditions; M.W. (theoretical): 46 kDa (monomer)
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.
Mouse myeloma cell line, NS0-derived mouse CD40/TNFRSF5 protein Mouse CD40 (Val24-Arg193) (N-terminus) Accession # P27512 DIEGRMD Human IgG1 (Pro100-Lys330) 6-His tag (C-terminus)
Disulfide-linked homodimer
Cd40
Yes
CD40/TNFRSF5
Bioactivity
958
CD40/TNFRSF5 Fc Chimera
50 µg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
<0.10 EU / 1 µg of the protein by the LAL method.
Unconjugated
Reconstitute at 100 µg/mL in sterile PBS.
SDS
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