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R&D Systems™ Recombinant Mouse OX40 Ligand/TNFSF4 Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 1236OX025

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Catalog No. 1236OX025

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Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 10-30 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ).


Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
M.W. (Observed): 21-25 kDa, reducing conditions; M.W. (theoretical): 18 kDa
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Mouse myeloma cell line, NS0-derived mouse OX40 Ligand/TNFSF4 protein Gln49-Leu198, with an N-terminal 10-His tag
<1.0 EU / 1 µg of the protein by the LAL method.
Reconstitute at 10 µg/mL in sterile PBS containing (at least 0.1% human or bovine serum albumin.)
OX40 Ligand/TNFSF4
25 µg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
OX40 Ligand/TNFSF4


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