R&D Systems™ Recombinant Mouse OX40 Ligand/TNFSF4 Protein, CF
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Manufacturer: R&D Systems™ 1236OX025/CF
DescriptionMeasured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 10-30 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ).
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|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.|
|Lyophilized from a 0.2 µm filtered solution in PBS.|
|M.W. (Observed): 21-25 kDa, reducing conditions; M.W. (theoretical): 18 kDa|
|>95%, by SDS-PAGE under reducing conditions and visualized by silver stain|
|Mouse myeloma cell line, NS0-derived mouse OX40 Ligand/TNFSF4 protein Gln49-Leu198, with an N-terminal 10-His tag|
|<1.0 EU / 1 µg of the protein by the LAL method.|
|Reconstitute at 100 µg/mL in sterile PBS.|
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