R&D Systems™ Recombinant Mouse OX40 Ligand/TNFSF4 Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 1236OX025/CF

Catalog No. 1236OX025CF

Description

Measured by its ability to co-stimulate IL-2 secretion by mouse T cells in the presence of anti-CD3. The ED50 for this effect is 10-30 ng/mL in the presence of a cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050 ).

Specifications

• Accession Number: P43488
• Formulation: Lyophilized from a 0.2 µm filtered solution in PBS.
• Molecular Weight: M.W. (Observed): 21-25 kDa, reducing conditions; M.W. (theoretical): 18 kDa
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Source: Mouse myeloma cell line, NS0-derived mouse OX40 Ligand/TNFSF4 protein Gln49-Leu198, with an N-terminal 10-His tag
• Endotoxin Concentration: <1.0 EU / 1 µg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7292
• Name: OX40 Ligand/TNFSF4
• Quantity: 25 µg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Gene Symbol: Tnfsf4
• Recombinant: Yes
• Protein: OX40 Ligand/TNFSF4