R&D Systems™ Recombinant Mouse PD-1 Fc Chimera Protein

Extensive quality control produces lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Binding Activity

Supplier:  R&D Systems™ 1021PD100

Catalog No. 1021PD100

Description

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse PD-1 Fc Chimera at 1 μg/mL (100 μL/well) can bind Recombinant Mouse B7-H1/PD-L1 Fc Chimera (Catalog # 1019-B7 ) with an ED50 of 0.16-0.8 μg/mL.

Specifications

• Accession Number: Q02242
• Formulation: Lyophilized from a 0.2 μm filtered solution in Tris-HCL, NaCl and EDTA with Trehalose.
• Molecular Weight (g/mol): M.W. (Observed): 66 kDa, reducing conditions; M.W. (theoretical): 43 kDa (monomer)
• Quantity: 100 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Gene Symbol: Pdcd1
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.

• For Use With (Application): Binding Activity
• Gene ID (Entrez): 5133
• Name: PD-1 Fc Chimera
• Source: Mouse myeloma cell line, NS0-derived mouse PD-1 protein Mouse PD-1 (Leu25-Gln167) (N-terminus) Accession # Q02242 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Structural Form: Disulfide-linked homodimer
• Gene Alias: CD279, CD279 antigen, hPD-1, PD1, PD1hPD-l, PDCD1, programmed cell death 1, programmed cell death protein 1, Protein PD-1, SLEB2
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 0.5 mg/mL in sterile water.
• Protein: PD-1