R&D Systems™ Recombinant Mouse Thrombospondin-1 Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity

Supplier:  R&D Systems™ 7859TH050

Catalog No. 7859TH050

Description

Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. The ED50 for this effect is 0.1-0.5 μg/mL.

Specifications

• Accession Number: NP_035710
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 130-155 kDa, reducing conditions; M.W. (theoretical): 129 kDa
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Gene Symbol: Thbs1
• Recombinant: Recombinant
• Purity or Quality Grade: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining.

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 7057
• Name: Thrombospondin-1
• Source: Mouse myeloma cell line, NS0-derived mouse Thrombospondin-1 protein Mouse Thrombospondin-1 (Asn19-Ser1171) (N-terminus) Accession # NP_035710 GGGSGGGSGGGS 6-His tag (C-terminus)
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in PBS.
• Protein: Thrombospondin-1