R&D Systems Mouse Wnt-5b Recombinant Protein
Manufacturer: R&D Systems 3006WN025
DescriptionMeasured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED 50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt-3a (Catalog # 1324-WN ). Optimal dilutions should be determined by each laboratory for each application.
|Lyophilized from a 0.2μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.|
|Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution.|
|75%, by SDS-PAGE under reducing conditions and visualized by silver stain.|
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