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R&D Systems™ Recombinant Rat B7-1/CD80 Fc Chimera Protein, CF

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Manufacturer:  R&D Systems™ 1214B7050

Catalog No. 1214B7050

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Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.1-0.4 μg/mL in the presence of PHA.


Lyophilized from a 0.2 µm filtered solution in PBS.
M.W. (Observed): 80-90 kDa, reducing conditions; M.W. (theoretical): 50.5 kDa (monomer)
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Mouse myeloma cell line, NS0-derived rat B7-1/CD80 protein Rat B7-1 (Ile39 - Gln248) (N-terminus) Accession # AAB60503 IEGRMD Human IgG1 (Pro100 - Lys330) (C-terminus)
Disulfide-linked homodimer
B7-1/CD80 Fc Chimera
50 µg
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
<0.10 EU / 1 µg of the protein by the LAL method.
Reconstitute at 100 µg/mL in sterile PBS.
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