R&D Systems™ Recombinant Rat B7-2/CD86 Fc Chimera Protein

Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility.

Supplier:  R&D Systems™ 1340B2050

Catalog No. 1340B2050

Description

Measured by its ability to induce IL-2 secretion by Jurkat human acute T cell leukemia cells. Freeman, G.J. et al. (1993) Science 262:909. The ED50 for this effect is 0.075-0.3 μg/mL in the presence of PHA.

Specifications

• Accession Number: O35531
• Formulation: Lyophilized from a 0.2 μm filtered solution in PBS.
• Molecular Weight (g/mol): M.W. (Observed): 80-100 kDa, reducing conditions; M.W. (theoretical): 52 kDa (monomer)
• Quantity: 50 μg
• Storage Requirements: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution.
• Endotoxin Concentration: <0.10 EU / 1 μg of the protein by the LAL method.
• Conjugate: Unconjugated
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• Protein: B7-2/CD86

• For Use With (Application): Bioactivity
• Gene ID (Entrez): 942
• Name: B7-2/CD86 Fc Chimera
• Source: Mouse myeloma cell line, NS0-derived rat B7-2/CD86 protein Rat B7-2 (Val29-Ile250) (N-terminus) Accession # O35531 IEGRMD Human IgG1 (Pro100-Lys330) (C-terminus)
• Structural Form: Disulfide-linked homodimer
• Gene Symbol: Cd86
• Recombinant: Recombinant
• Purity or Quality Grade: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain