R&D Systems Recombinant Human Lymphotoxin-alpha/TNF-beta

Tumor Necrosis Factor beta

Manufacturer: R&D Systems 211TBB050/CF

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Catalog No. 21-1TB-B050CF

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    Specifications

    Accession Number P01374
    Conjugate Unlabeled
    Format Reconstitution: Reconstitute at 100μg/mL in PBS.
    Formulation Lyophilized from a 0.2μm filtered solution in PBS.
    Molecular Weight 19 kDa
    Name Lymphotoxin-alpha/TNF-beta, Carrier Free
    Purity >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Quantity 50μg
    Recombinant Yes
    Source E. coli-derived, Leu35-Leu205, with an N-terminal Met

    TNF-beta, also known as lymphotoxin-alpha (LTalpha) and TNF-alpha are two structurally and functionally related proteins that bind to the same cell surface receptors (TNF RI and TNF RII) and produce a vast range of similar, but not identical effects. Mature TNF-beta/LTalpha and TNF-alpha share approximately 35% protein sequence homology, and the biologically active secreted forms of both proteins are trimers. Human and mouse TNF-beta/LTalpha share approximately 74% homology in their amino acid sequence and exhibit cross-species activity. Soluble TNF-beta/LTalpha is a homotrimer in solution. Secreted TNF-beta/LTalpha also complexes with the membrane associated LTbeta/TNFSF3 to generate two types of heterotrimers, LTalpha1/beta2 and LTalpha2/beta1.

    N-terminal Sequence Analysis: Met
    SDS-PAGE: 17-19 kDa, reducing conditions
    Activity: Measured in a cytotoxicity assay using LG–929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.The ED50 for this effect is typically 4-20 pg/mL.

  • Description & Specifications

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